| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.12[luc2CP] |
| 质粒类型: | 荧光素酶载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 4421 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 编码高度不稳定荧光素酶的无启动子载体，用于测定启动子和增强子序列的活性。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
![pGL4.12[luc2CP]载体图谱](/uploads/载体图谱/pGL4.12[luc2CP]载体图谱.png)
载体简介
载体序列
LOCUS pGL4.12[luc2CP] 4421 bp ds-DNA circular SYN 29-JUN-2013
DEFINITION Promoterless vector encoding highly destabilized luciferase for
measuring the activity of promoter and enhancer sequences.
ACCESSION AY738224
VERSION .
KEYWORDS pGL4.12[luc2CP]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4421)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Saturday, June 13, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4421
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
CDS 100..1749
/codon_start=1
/product="firefly luciferase"
/note="luciferase"
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
CDS 1756..1803
/codon_start=1
/product="non-ORF yeast peptide conferring
ubiquitin-dependent degradation"
/note="hCL1"
/note="human codon-optimized"
/translation="ACKNWFSSLSHFVIHL"
CDS 1807..1926
/codon_start=1
/product="PEST degradation sequence from mouse ornithine
decarboxylase"
/note="hPEST"
/note="human codon-optimized"
/translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
polyA_signal 1975..2096
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2515..3103)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3303..4163)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 4268..4316
/note="synthetic polyadenylation signal"
misc_feature 4330..4421
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
1741 atcgccgtga attctgcttg caagaactgg ttcagtagct taagccactt tgtgatccac
1801 cttaacagcc acggcttccc tcccgaggtg gaggagcagg ccgccggcac cctgcccatg
1861 agctgcgccc aggagagcgg catggataga caccctgctg cttgcgccag cgccaggatc
1921 aacgtctaac tgcagtctag agtcggggcg gccggccgct tcgagcagac atgataagat
1981 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
2041 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
2101 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
2161 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg
2221 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
2281 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc
2341 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
2401 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
2461 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
2521 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
2581 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
2641 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
2701 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
2761 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
2821 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
2881 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
2941 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
3001 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
3061 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
3121 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
3181 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
3241 ctaaagtata tatgagtaaa cttggtctga cagcggccgc aaatgctaaa ccactgcagt
3301 ggttaccagt gcttgatcag tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc
3361 atagtggcct gactccccgt cgtgtagatc actacgattc gtgagggctt accatcaggc
3421 cccagcgcag caatgatgcc gcgagagccg cgttcaccgg cccccgattt gtcagcaatg
3481 aaccagccag cagggagggc cgagcgaaga agtggtcctg ctactttgtc cgcctccatc
3541 cagtctatga gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag tagtttccga
3601 agagttgtgg ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg
3661 ttcaactctg gttcccagcg gtcaagccgg gtcacatgat cacccatatt atgaagaaat
3721 gcagtcagct ccttagggcc tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg
3781 ctcatggtaa tggcagcact acacaattct cttaccgtca tgccatccgt aagatgcttt
3841 tccgtgaccg gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc
3901 tgctcttgcc cggcgtctat acgggacaac accgcgccac atagcagtac tttgaaagtg
3961 ctcatcatcg ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc gctattgaga
4021 tccagttcga tatagcccac tcttgcaccc agttgatctt cagcatcttt tactttcacc
4081 agcgtttcgg ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg aatgagtgcg
4141 acacgaaaat gttggatgct catactcgtc ctttttcaat attattgaag catttatcag
4201 ggttactagt acgtctctca aggataagta agtaatatta aggtacggga ggtattggac
4261 aggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
4321 gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata
4381 ggctgtcccc agtgcaagtg caggtgccag aacatttctc t
//
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